A novel method for the determination of protonation constant

S SINGH, S R TRIPATHI, M M TIWARI, P C YADAVA, K L Yadava

Abstract


It is for the first time that the use of paper electrophoresis is described for the assessment of proton at ion constants. The method is based on migration of a spot of the peptide with variation of pH of background electrolyte containins 0.1 M perchloric acid. The graph between pH vs mobility gives information about nature of dissociation and help in calculating them. The calculated values are fairly in agreement with literature values.

Keywords


Paper electrophoresis; protonation constants; mobility.

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