CIRCULAR PAPER CHROMATOGRAPHY. I. A Technique for tile Separation and Identification of Amino Acids

Abstract

1. A  technique  for  the  separation  and  identification  of  amino  acids  on  partition  chromatograms  using  circular  filter-paper  discs  as  the  stationary  phase  is  described.  N-butanol-acetic  acid-water  has  been  used  as  the  developing  solvent.
2. The  method  of  funning  mixed  chromatograms  for  the  identification  of  amino  acids  has  been  described.
3. Multiple  development  technique  is  found  to  be  a  very  useful  technique  for  achieving  clear-cut  separation  of  amino  acids  which  do  not  readily  separate  into  distinct  bands  by  first  development.
4. A  scheme  for  the  qualitative  analysis  of  amino  acids  has  been  indicated.
5. The  advantages  of  this  technique  over  the  other  widely  used  paper  chromatographic  techniques  have  been  discussed  and  the  possibilities  of  the  application  of  this  technique  in  other  fields  of  research  are  indicated.