A NOTE ON THE ELECTROPHORETIC PATTERN OF SERUM PROTEINS IN ACUTE HAEMOLYTIC ANAEMIA OF RATS

Abstract

Phenylhydtazine in 12 mg./kg dose induces severe hemolytic anamia associated with pathological involvement of liver, spleen and other organs, simulating those observed in acute experimental malaria. Since the etiological factors and the sequence of events involved in the hiemolysis are different, a study of biochemical changes in this condition has been carried out and compared with those observed in other toxic and hamolytic states.Electrophoretic studies on rat serum proteins in pbenyl-hydrazine induced hiemolytic anamia of rats indicate practically no alteration in total serum protein but appreciable increase in y globulin and a slight decrease in albumin. No marked changes in a,, az and p globulins and no new componenb have been observed. Mobilities do not show any change in this pathological state.Since no infective or antigenic stimulation is involved, no explanation can be offered for the large increase in the y globulin fraction observed in the h~molytic ansmia.