AGAR ELECTROPHORESIS Part VI. Lipoproteins

Abstract

Agar electrophoresis technique as described by Giri has been applied for thestudy of separation and estimation of serum lipoproteins in normal and pathologicalcases such as nephritis. diabetes, myxoedema and hypertensive heart diseases and in a few animals viz., rat, rabbit, guinea pig, sheep. goat, cow and buffalo.There are atleast two lipoprotein components revealed by staining with 0.1 percent Sudan Black in 60 per cent ethanol in normal as wen as in pathological humanserum samples. The fast moving component has the mobility of serum albumin and the slow moving component has the mobility of Xl globulin. They occupy about 30 per cent and 70 per cent of the total sudanophiliic area respectively. In pathological cases there is a relative increase in the slow moving lipoprotein component.