Synthesis of new immunogens for the development of radioimmunoassay of levonorgestrel and its 3-oximes

Abstract

The synthesa, from 13beta-ethyl-17beta-hydroxy-3-methoxygona- 1,3,5(10),8 tetraene,of new immunogens for levonorgestrel and its 3-syn and antz-oximes by coupling the steroids to bovine serum albumin (BSA) via an 11 beta-carboxymethoxymethyl group is reported. In addition, the preparation of homologous bistamine conjugates, which serve as substrates for the synthesis of '"I-labelled ligands required for radioimmunoassay (RIA), a reported. The Ilp-carboxymethoxymethyl group was constructed through an R$(OAC) catalyzed carbene msertion reaction into the hydroxyl bond of an llp-hydroxpethyl derivative