Spectrophotometric studies of copper-streptomycin complex and determination of streptomycin sulphate using an auxiliary ligand.

Abstract

Spectrophotonletric investigation of the deep blue, water soluble complex of streptomycin sulphate with bivalent copper in the alkaline medium has been carried out in presence of an auxiliary ligand sodium potassium tartrate. The complex (at p11 8 .5) is found to absorb at 650 nm. Job's method and the mole ratio method have shown the composition of the complex to be 1: 1. Conditional stability constant has been determined by Mukherjee and Dey method and Raghav Rao's method. The log K, value by these two methods is found to be 3.98 and 3-93 respectively.

The complex obeys Beer's law in the range of 0. 25 to 5 mg streptomycin sulphate/ml of the solution. The technique is found to be suitable for the quantitative estimation of streptomycin.