THE EFFECT OF AMlNOACIDS ON GROWTH, SPORULATION AND CRYSTAL FORMATION IN BACILLUS THURINGIENSIS VAR. THURINGIENSIS*

S RAJALAKSHMI, Y I SHETHNA

Abstract


Histidine, threonin, valine,  tyrosine,  leucine,  isolucine,  serine,  lysine  and  B  vitamins added  to  Glucose-mineral  salts medium  did  not  promote growth  of  Bacillus thuringiensis.  Aspartic  acid, arginine,  glycine,  proline,  asparagine,  methionine  and  glutamine supported  growth  of  the  organism  with a  longer lag  period  as  compared  to  cystine,  which  stimulated  exponential growth in  9  hr  after  inoculation.  The total  growth  increased  in  relation  to  the  concentration  of  cystine  in the medium.  Presence  of  excess  cystine  along  with  glucose inhibited  sporulation  and  consequent  parasporal  crystal  formation.


Keywords


Aminoacids; growth; sporulation; Bacillus thuringiensis.

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