CIRCULAR PAPER CHROMATOGRAPHY Part III. Rf Values of Amino Acids and Peptides.

Abstract

1. The Rf  values on circular  filter-paper chromatograms  of a number of amino acids in various solvents have been determined. The follow-  ing solvent mixtures have been studied: n-Butanol-acetic acid-water,  phenol water, mesityl oxide-formic acid and aqueous acetone.
2. The influence of concentration of amino acids, the distance  of the solvent boundary and the distance of the initial spot from the centre  of the filter-paper, and temperature on the Rf  values have been studied.
3. The Rf  values of some of the peptides in n-butanol-water solvent  mixture are given.
4. The influence of various factors involved in the movement of  amino acids on circular paper chromatograms and the mechanism of  chromatographic separation of the amino acids in the light of the results  obtained in the present investigation are discussed.