CIRCULAR PAPER CHROMATOGRAPHY PART- V :—The separation, identification and quantitative determination of urea with application to human urine.

Abstract

A method for the separation, identification and quantitative determination of  urea by means of circular paper chromatography is described. Samples to be tested  are chromatographed, the position of urea on the chromatogram is indicated  by spraying one half of the paper with phenol-hypochlorite reagent. The urea  band on the unsprayed half of the chromatogram is cut out, eluted with water and  the urea is determined colorimetrically with a-isonitrosopropiophenone reagent.

The method has been applied to the determination of urea in urine.  It  is  obvious that the method in principle can also be used for the analysis of other  constituents in urine.