RecA protein-promoted homologous pairing and strand exchange in vitro: A model to study the molecular mechanism(s) of gene targeting

Abstract

Homologous genetic recombination is ubiquitous. The insight(s) into the cascade of events that culminate in the productlon of heteroduplex DNA emanated from the studies done, in vitro, using the E.coil RecA protein paradigm. Our pioneering studies to understand the role of structural features of the E. coli and cukaryotic chromosomes on the mechanistics of homologous recombination, in vitro, and to evolve strategies for robust gene targeting has been discussed