AN ELECTROPHORETIC STUDY OF BOVINE PLASMA ALBUMIN--METHYL ORANGE COMPLEX AT 25° C.

Abstract

Kern's micro-electrophoresis apparatus has been employed to study the interaction between bovine plasma albumin (1.49x 10 -4 M) and  methyl orange (1.53.25.0 x 10 -4M) M) at the laboratory temperature (25° C.) using acetate buffer of ionic strength 0.05 and pH 5-57. The dissociation constant of the protein-dye complex has been found to be 3.6x 10-4 while the maximum number of dye molecules combined permolecule of protein is 20. In contrast to observations given in literature,the electrophoretic patterns do not show any asymmetry in the complex boundary at higher dye concentrations.