A MICRO-ELECTROPHORETIC STUDY OF THE COMPLEX FORMATION OF BOVINE PLASMA ALBUMIN (B.P.A.) AND RIBOSE NUCLEIC ACID (R.N.A.).

Abstract

The complex formation of B.P.A./R.N.A. system has been studied at various pH, ionic strengths and concentration ratios. It has been found that complex is formed only below pH 5.60 and pi = .1. The ionic strength is critical for the formation of the complex as only two boundaries are obtained in both the limbs at .02 p, while at 0512 three boundaries are obtained in the descending limb and two in the ascending limb consistently.The results have been discussed by modified Jordan's equation to explain the occurrence of third boundary in the descending limb.Calculations made by the method of Briggs et al. have failed to yield the value of reaction constants n and k. Similar calculations with data of Goldwasser and Putnam have given the value of n = -024 and k = 26x 10-5 . The data of Kirkwood also fails to give any constants.