Deviation behaviour of charged and aromatic molecules on highly cross.linked dextran and polyacrylamide gels

Abstract

Giucnronic acid and glutamic acid were excluded more strongly from Bio-Gel P-2, than from Sephadex 0-15. Lysine and arginine were more strongly retarded on Bio-O,1 P-2. Phosphate buffer (pH 7'0,0'05 M) was found to be superior to 0'05 M Tris-HCI and 0'05 MNaCI solutions in suppressing electrostatic interaction on these gels. Complete normalIZation of the deviation patterns of charged molecules on these gels could not be achieved in all cases with different salt solutions as eluants. Arginine and lysine could be separated due to their differing degree of retention on Sephadex G-15 and Bio-Hel P-2. Retention of aromatic compounds was stronger on the cross--linked dextran gel.