Comparative study ofthe action of cereal protease inhibitors on human, bovine and porcine pancreatic enzymes

Abstract

Extract  of  corn, barley,  ragi,  sorghum, pearl  millet,  setaria,  echinocloa  and kodo  seeds  were  tested  for  inhibitory  activity  against  human and  bovine  Pancreatic  proteases.  Setaria  was equally  actives against the  two  systems.  Kodo  was more effective in  inhibiting  the caseinolytic trypric (hydrolosis of  benzoyl arglmine p-nitrtoanilide-BAPNA)and chymotryptic  (hydrolysis  of  acetyl  tyrosine  ethyl  ester-ATEE)  activities  of  the  human  pancreatic preparation  whereas corn,  barley  and  echinocloa  were  potent  against  the bovine  system.  Other  seeds showed  mixed  patterns. Inhibitory activity against  clastase  (hydrolysis  of  succinyl  trialanyl  p-nilroanilide-STANA)  activity  in  bovine  pancreatic  extract  was  relatively  poor  in all the  seeds.  Comparative  studies  on  the  inhibition  of crystallized porcine  elastase  with  casein and  STANA  as substrates shawed that  magnitude of inhibition was more (1.70 fold in barley to 13.2  fold  in pearl  millet) with  all  the  seeds  when  casein  was  used.  Similar studies with  crystalline  bovine  trypsin  and  chymotrypsin revealed significant difference onlv  with  kodo which  was  found  to  be eight times more powerful in blocking tryptic activity when BAPNA was used as substrate.