GENETIC STUDIES IN MYCOBACTERIA: ISOLATION OF AUXOTROPHS AND MYCOBACTERIOPHAGES FOR MYCOBACTERIUM SMEGMATIS AND THEIR USE IN TRANSDUCTION

C V SUNDAR RAJ, T RAMAKRISHNAN

Abstract


Auxotrophs of Mycobacterium smegmatis SN2 have been isolated using ultraviolet irradiation and N-methyl-N'-nitro- N-nitrosoguanidine as mutagens. Ten mycobacteriophages have been isolated from soil. One of these phages, I3 has been shown to be a generalised transducing phage. Transduction has been found to be sensitive to antiphage serum and insensitive to  deoxyribonuclease The host-range of the phages have been studied on several related mycobacteria species.

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