MICROBIAL PRODUCTION OF AMINO ACIDS III. NUTRITIONAL STUDIES AND THE EFFECTS OF ORGANIC GROWTH PROMOTERS AND THIAMINE ON DL-ALANINE FERMENTATION BY ARTHRO-BACTER STRANIN C19d

Abstract

The study of the nutritional requirements of Arthrobacter strain C₁₉d which accumulates alanine in large amounts in the culture medium. revealed that the organism needs thiamine for its growth. Also the alanine accumulation by this strain was found to be related to thiamine concentration in the medium. The optimum concentration of thiamine for alanine accumulation(20 p.gjml) was also optimum for the growth of the organism indicating there by that alanine accumulation by this strain is a growth associated process rather than far removed from it. Among the various growth promoters tried yeast extract was found to be superior from the point of view of alanine yield and it was also superior to giving thiamine alone in the medium. A concentration 0/0.02% yeast extract was found to be optimum for alanine occumulation.