THE REACTION OF LIVING VEGETATIVE CELLS AND ZYGOTES OF SACCHAROMYCES CARLSBERGENSIS TO NEUTRAL RED.

Abstract

1. The reaction of living vegetative cells from 24-hr. agar slants to different concentrations of neutral red has been investigated. Guilliermond's repeated emphasis that the vacuole is not the nucleus of yeast and that neutral red is specific for the study of the vacuole of living cells is confirmed and illustrated with photomicrographs.

2. A comparison of the reaction of living vegetative cells and zygotes without and with visible nuclei shows that neutral red produces neo-formations only inside the vacuole, thus emphasizing the unrelated nature of the vacuole and the nucleus.