Separation of protocatechuate and homoprotocatechuate by paper chromatography and estimation of protocatechuate by colorimetry using a new FeCI3 reagent

Satya Narayana Ganapathi Raju, Nayeem U Khan, C S VidhyaNathan

Abstract


A new chromogenic reagent for orthodihydroxy phenolic compounds is prepared and its use in detection of protocatechuic acid, homoprotocatechuic acid and protocatechuic aldehyde after separation by pair chromatography is described. The reagent is also useful for the colorimetric estimation of protocatechuic acid.

Keywords


Paper chromatography; protocatechuate; homoprotocatechuate; colorimetry.

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