EFFECT OF NH4+ ON ACETYLENE REDUCTION (NITROGENASE) IN AZOTOBACTER VINELANDII AND BACILLUS POLYMYXA*

P T CHANDRASEKHARAN NAMBIAR, Y I SHETHNA

Abstract


In  vivo  acetylene reduction  (nitrogenase)  is  inhibited  by  NH4+  immediately  in  Azotobacter  vinelandii,  but not  in  Bacillus  polymyxa.  In  addition  to  the  repression  of  enzyme synthesis,  NH4+  has  two  types  of  inhibitory effects  on  acetylene  reduction  in  A.  vinelandii  (i)  reversible  inhibition  in  the  initial  stages  of incubation with NH4+,  (ii)  irreversible loss  of  activity in  the  later stages of  incubation  with  NH4+


Keywords


Ammonia; nitrogenase; Azotobacter; Bacillus polymyxa.

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