PROBLEMS IN THERMOPHILY - IV. The Nutritional Requirements of Some Thermophilic Bacilli.

Abstract

Screening of 80 isolates of the various species of bacilli growing at 55' C. revealed that none of them could grow at that temperature in a medium containing only an inorganic source of nitrogen. Twenty-seven isolates were able to grow in the same medium at 37° C. Addition of biotin did not promote growth of those that failed to grow at either temperatures. A closer examination of two strains of B. coagulans and one of B. break indicated that the sulphur-containing amino acids constituted essential amino acids for them to grow.